Journal: Journal of Nanobiotechnology

Article Title: Cerium-doped prussian blue nanozymes for bone regeneration and osteoprotection by Lhx2 in sutural distraction osteogenesis through multi-omics analysis

doi: 10.1186/s12951-025-03931-9

Figure Lengend Snippet: Systematical observation and analysis of bone remodeling and regeneration within the mid-palatal suture expansion by histological and immunohistochemical staining. ( A ) HE and Masson’s trichrome staining. ( B ) TRAP staining and IHC staining of OCN, CD31, RANKL and OPG expression. Black arrows indicate osteoclasts in mid-palatal suture. ( C ) The number of osteoclasts per square millimeter was quantified through TRAP staining. H-Score of ( D ) OCN, ( E ) CD31, ( F ) RANKL and ( G ) OPG. ( H ) The relative value of H-Score of RANKL to OPG (* p < 0.05, ** p < 0.01, *** p < 0.001 and ns means no significance). H -Score: histochemistry score

Article Snippet: Rat M-CSF recombinant protein was purchased from PeproTech ® , USA and Rat RANKL recombinant protein was purchased from R&D Systems, USA.

Techniques: Immunohistochemical staining, Staining, Immunohistochemistry, Expressing

Journal: Journal of Nanobiotechnology

Article Title: Cerium-doped prussian blue nanozymes for bone regeneration and osteoprotection by Lhx2 in sutural distraction osteogenesis through multi-omics analysis

doi: 10.1186/s12951-025-03931-9

Figure Lengend Snippet: Systematical observation and analysis of bone remodeling and regeneration within the mid-palatal suture excessive expansion by histological and immunohistochemical staining. ( A ) HE and Masson’s trichrome staining. (B ) TRAP staining and IHC staining of OCN, CD31, RANKL and OPG expression. Black arrows indicate osteoclasts in mid-palatal suture. ( C ) The number of osteoclasts per square millimeter was quantified through TRAP staining. H-Score of ( D ) OCN, ( E ) CD31, ( F ) RANKL and (G ) OPG. ( H ) The relative value of H-Score of RANKL to OPG (* p < 0.05, ** p < 0.01, *** p < 0.001 and ns means no significance). H-Score: histochemistry score

Article Snippet: Rat M-CSF recombinant protein was purchased from PeproTech ® , USA and Rat RANKL recombinant protein was purchased from R&D Systems, USA.

Techniques: Immunohistochemical staining, Staining, Immunohistochemistry, Expressing

Journal: Cureus

Article Title: Elevation of NTN4 Expression and Its Possible Regulation by Tumor Necrosis Factor-Alpha (TNF-α) in a Rat Model of Rotator Cuff Tear

doi: 10.7759/cureus.86324

Figure Lengend Snippet: Box-and-whisker plots depict mRNA expression levels of (A) Ntn4, (B) Il6, (C) Tnfa, (D) Mmp1, (E) Mmp3, and (F) Cxcl1 in tendon tissue at 0 days (intact control) and at 7, 14, 28, and 56 days post-injury. Statistical significance (*) is indicated for comparisons versus day 0 (P < 0.05). Statistical analysis was performed using the Kruskal–Wallis test with Dunn's post-hoc test.

Article Snippet: TNF-α and NTN4 treatment After serum starvation in α-MEM containing 1% FBS for 12 hours, tenocytes were exposed either to recombinant rat NTN4 (R&D Systems, Minneapolis, MN, USA) at final concentrations of 0, 50, or 500 ng/mL or to recombinant rat TNF-α (PeproTech, Cranbury, NJ, USA) at 0, 1, or 10 ng/mL.

Techniques: Whisker Assay, Expressing, Control

Journal: Cureus

Article Title: Elevation of NTN4 Expression and Its Possible Regulation by Tumor Necrosis Factor-Alpha (TNF-α) in a Rat Model of Rotator Cuff Tear

doi: 10.7759/cureus.86324

Figure Lengend Snippet: mRNA expression levels of (A) Ntn4, (B) Il6, (C) Tnfa, (D) Mmp1, (E) Mmp3, and (F) Cxcl1 were measured in primary tenocytes isolated from rat rotator cuff tendons following stimulation with recombinant NTN4. Data are presented as mean ± SEM. Statistical significance (*) is indicated for comparisons versus control (0 ng/mL) (P < 0.05). Statistical analysis was performed using the Kruskal–Wallis test with Dunn's post-hoc test. SEM: standard error of the mean

Article Snippet: TNF-α and NTN4 treatment After serum starvation in α-MEM containing 1% FBS for 12 hours, tenocytes were exposed either to recombinant rat NTN4 (R&D Systems, Minneapolis, MN, USA) at final concentrations of 0, 50, or 500 ng/mL or to recombinant rat TNF-α (PeproTech, Cranbury, NJ, USA) at 0, 1, or 10 ng/mL.

Techniques: Expressing, Isolation, Recombinant, Control

Journal: Cureus

Article Title: Elevation of NTN4 Expression and Its Possible Regulation by Tumor Necrosis Factor-Alpha (TNF-α) in a Rat Model of Rotator Cuff Tear

doi: 10.7759/cureus.86324

Figure Lengend Snippet: Protein concentrations of (A) IL-6 and (B) MMP-3 were measured by ELISA in culture supernatants of rat rotator cuff-derived tenocytes following stimulation with recombinant NTN4. Data are presented as mean ± SEM. Statistical significance (*) is indicated for comparisons versus control (0 ng/mL) (P < 0.05). Statistical analysis was performed using the Kruskal–Wallis test with Dunn's post-hoc test. ELISA: enzyme-linked immunosorbent assay; SEM: standard error of the mean

Article Snippet: TNF-α and NTN4 treatment After serum starvation in α-MEM containing 1% FBS for 12 hours, tenocytes were exposed either to recombinant rat NTN4 (R&D Systems, Minneapolis, MN, USA) at final concentrations of 0, 50, or 500 ng/mL or to recombinant rat TNF-α (PeproTech, Cranbury, NJ, USA) at 0, 1, or 10 ng/mL.

Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Recombinant, Control

Journal: Cureus

Article Title: Elevation of NTN4 Expression and Its Possible Regulation by Tumor Necrosis Factor-Alpha (TNF-α) in a Rat Model of Rotator Cuff Tear

doi: 10.7759/cureus.86324

Figure Lengend Snippet: mRNA expression levels of Ntn4 were measured in rat rotator cuff-derived tenocytes following stimulation with TNF-α. Data are presented as mean ± SEM. Statistical significance (*) is indicated for comparisons versus control (0 ng/mL) (P < 0.05). Statistical analysis was performed using the Kruskal–Wallis test with Dunn's post-hoc test. SEM: standard error of the mean; TNF-α: tumor necrosis factor-alpha

Article Snippet: TNF-α and NTN4 treatment After serum starvation in α-MEM containing 1% FBS for 12 hours, tenocytes were exposed either to recombinant rat NTN4 (R&D Systems, Minneapolis, MN, USA) at final concentrations of 0, 50, or 500 ng/mL or to recombinant rat TNF-α (PeproTech, Cranbury, NJ, USA) at 0, 1, or 10 ng/mL.

Techniques: Expressing, Derivative Assay, Control